Fast and flexible - Crack your protein expression challenges      


RTS is a scalable in vitro transcription/translation protein expression platform, that produces sufficient amounts of protein for characterization studies, functional assays, or structural analysis. RTS offers the advantages of a cell-free protein expression system, eliminating the need for laborious up- and down-stream steps (e.g. host cell transformation, culturing, or lysis) typically associated with cell-based expression systems. Two modular systems are available: the prokaryotic E. coli or the eukaryotic Wheat Germ.
 
For both lines, screening (RTS 100) as well as scale-up (RTS 500, RTS 9000) kits are provided.
 
Generally, to further fasten the protein expression screening, we recommend to use linear (PCR-generated) templates with the RTS screening kits. These templates are produced via the RTS Linear Template Generation Sets.
For scale-up, the use of circular templates is recommended, which are generated with the RTS pIVEX Vector Sets. In order to yield mg quantities, expressions should be performed using the RTS ProteoMaster instrument. (One instrument for all kit formats and both lines.)
 
For optimization of the prokaryotic Rapid Translation, we offer a special software, the ProteoExpert RTS E. coli. The software delivers high-yield variants of an input ORF sequence.