Synthesis of recombinant proteins in high yields
EasyXpress Protein Synthesis Maxi Kit
- Fast procedure - go from gene to protein in a single day
- High yields - up to 1 mg protein per ml reaction volume in just one hour
- A convenient procedure - color-coded reagents, and easy-to-follow protocols
- Easily scalable reactions with reproducible yields – suitable also for expression screening in small volumes (>5 µl)
- For reactions up to 4000 µl: 4 x 350 µl E. coli extract, reaction buffer, RNase-free water, positive-control DNA and handbook
Large-scale in vitro synthesis of recombinant proteins for structural studies
EasyXpress Protein Synthesis Mega Kit
- High yields of high-quality protein - one kit delivers enough protein for a comprehensive structural characterization
- Fast procedures - up to 5 mg purified protein per reaction in just 4 hours, compared to weeks using conventional methods
- Easy incorporation of selenomethionine - facilitating structural analyses using crystallography
- For 2 x 5 ml reactions: E. coli extract, reaction buffers, amino acid mix w/o methionine, methionine, RNase-free water, reaction flasks, gel-filtration columns and handbook
EasyXpress NMR Protein Synthesis Kit
- High yields of high-quality protein - one kit delivers enough protein for a comprehensive structural characterization
- Fast procedures - up to 5 mg purified protein per reaction in just 4 hours, compared to weeks using conventional methods
- Easy incorporation of selected isotopically labeled amino acids - facilitating structural analyses using NMR
- For 2 x 5 ml reactions: E. coli extract, reaction buffers, amino acid mix w/o Arg, Lys, Ser, Thr, Val (supplied separately as individual amino acids), RNase-free water, reaction flasks, gel-filtration columns, and handbook
- alternatively amino acid mix w/o single individual amino acids, e.g. w/o Ala (to be supplied by user) instead of omitting five amino acids Arg, Lys, Ser, Thr, Val in the amino acid mix
