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1. Cell-free Protein Expression

Serwa R., Wilkening I., Del Signore G., Mühlberg M., Claußnitzer I., Weise C., Gerrits M., Hackenberger C. (2009) Chemoselective Staudinger-Phosphite Reaction of Azides for the Phosphorylation of Proteins. Angew Chem Int Ed 48, 1 - 6 [Read Article]

Kubick S., Gerrits M., Merk H., Stiege W., Erdmann V.A. (2009)
In vitro Synthesis of posttranslationally modified Membrane Proteins
In: ’Current Topics In Membranes Vol. 63, Elsevier’, Chapter 2 [Abstract] [Amazon]

Katranidis A., Atta D., Schlesinger R., Nierhaus K., Choli-Papadopoulou T., Gregor I., Gerrits M., Büldt G. Fitter J. (2009) Fast Biosynthesis of GFP Molecules: A single-Molecule Fluorescence Study. Angew Chem Int Ed 48, 1758-1761 [ReadArticle]

Gerrits M., Claußnitzer I. (2008) Immobilization of site-specifically biotinylated proteins: Streptavidin vs. Strep-Tactin. QIAGEN News 2008 e16.  [PDF]

QIAGEN GmbH (2008) EasyXpress E. coli lysates - the fifth generation. QIAGEN News 2008 e16.  [PDF]

Gerrits M., Strey J., Claußnitzer I., von Groll U., Schäfer F., Rimmele M., Stiege W. (2007) Cell-free Synthesis of Defined Protein Conjugates by Site-directed Cotranslational Labeling. In: Kudlicki T., Katzen F., Bennett R. (Eds.) Cell-free Expression. Landes Bioscience, Austin. [Full Text]

Gerrits M., Kubick S., Merk H., Strey J., Stiege W. (2007) In vitro Synthesis of modified Proteins. In: Kyriakopoulos A., Michalke B., Graebert A., Behne D. (Eds.) Proceedings of the 5th Fall Conference on Metallobiolomics. Herbert Utz Verlag.

von Groll U., Kubick S., Merk H., Stiege W., Schäfer F. (2007) Advances in Insect-based Cell-free Protein Expression. In: Kudlicki T., Katzen F., Bennett R. (Eds.) Cell-free Expression. Landes Bioscience, Austin.  [Abstract]

Lamla T., Hoerer S., Bauer M.M. (2006) Screening for soluble expression constructs using cell-free protein synthesis. Int J Biol Macromol. 39(1-3), pp 111-121.  [PubMed]

Kubick S., Merk H., Stiege W., von Groll U., Drees J., Schaefer F. (2005) Insect-cell based in vitro synthesis of posttranslationally modified proteins. Qiagen News 2005 (2), pp 41-43.  [PDF]

Gerrits M., Strey J., Stiege W., von Groll U., Drees J., Schaefer F. (2005) Efficient large-scale synthesis and co-translation biotin labeling of recombinant proteins. Qiagen News 2005 (2), pp 38-40.  [PDF]

Royall E., Woolaway K.E., Schacherl J., Kubick S., Belsham G.J., Roberts L.O. (2004) The Rhopalosiphum padi virus 5' IRES is functional in Spodoptera frugiperda 21 cells and in their cell-free lysates; implications for the baculovirus expression system. J Gen Virol. 85(Pt 6), pp 1565-1569.  [Full Text]

Kubick S., Schacherl J., Fleischer-Notter H., Royall E., Roberts L.O., Stiege W. (2003) In Vitro Translation in an Insect-Based Cell-Free System. In: Swartz JR (Ed.) Cell-Free Protein Expression. Springer, Berlin, Heidelberg, New York, pp 209-217.

Merk H., Meschkat D., Stiege W. (2003) Expression-PCR: from Gene Pools to Purified Proteins Within 1 Day.  In: Swartz J.R. (Ed.) Cell-Free Protein Expression. Springer, Berlin Heidelberg New York, pp 15-23.

Lamla T., Stiege W., Erdmann V.A. (2002) An improved protein bioreactor: efficient product isolation during in vitro protein biosynthesis via affinity tag.  Mol Cell Proteomics. 1(6), pp 466-471.  [PubMed]

Szkaradkiewicz K., Nanninga M., Nesper-Brock M., Gerrits M., Erdmann V.A., Sprinzl M. (2002)  RNA aptamers directed against release factor 1 from Thermus thermophilus.  FEBS Lett. 514(1), pp 90-95.  [PubMed]

Gerrits M., Merk H., Stiege W., Erdmann V.A. (1999) Towards improved Applications of Cell-free Protein Biosynthesis – The influence of mRNA Structure and Suppressor tRNAs an the Efficiency of the System. In: Barciszewski J., Clark B.F.C. (Eds.) RNA Biochemistry and Biotechnology, NATO Science Series, Kluwer Academic Publishers, Dordrecht, Vol 70, pp 335-345.

Merk H., Stiege W., Tsumoto K., Kumagai I., Erdmann V.A. (1999) Cell-free Expression of two Single-Chain Monoclonal Antibodies against Lysozyme: Effect of Domain Arrangement on the Expression. J Biochem. 125(2), pp 328-333.  [PubMed]

Fuchs U., Stiege W., Erdmann V.A. (1997) Ribonucleolytic activities in the Escherichia coli in vitro translation system and in its separate components. FEBS Lett. 414(2), pp 362-364.  [PubMed]

2. Functional RNA

Schütze T., Arndt P.F., Menger M., Wochner A., Vingron M., Erdmann V.A., Lehrach H., Kaps C., Glökler J. (2010) A calibrated diversity assay for nucleic acid libraries using DiStRO—a Diversity Standard of Random Oligonucleotides. Nucleic Acids Res. 38(4), e23, doi:10.1093/nar/gkp1108. [PubMed] [PDF]

Menger M. (2009) Aptamere - Generierung und Applikation. GenomXPress 1.09, pp 17-19.  [PDF]

Ehrentreich-Forster E., Orgel D., Krause-Griep A., Cech B., Erdmann V.A., Bier F., Scheller F.W., Rimmele M. (2008) Biosensor-based on-site explosives detection using aptamers as recognition elements. Anal Bioanal Chem. 391(5), pp 1793-1800.  [PubMed]

Wochner A., Menger M., Orgel D., Cech B., Rimmele M., Erdmann V.A., Glokler J. (2008) A DNA aptamer with high affinity and specificity for therapeutic anthracyclines. Anal Biochem. 373(1), pp 34-42.  [PubMed]

Wochner A., Menger M., Rimmele M. (2007) Characterisation of aptamers for therapeutic studies. Expert Opin Drug Discov. 2(9), pp 1205-1224.  [Abstract]

Wochner A., Cech B., Menger M., Erdmann V.A., Glokler, J. (2007) Semi-automated selection of DNA aptamers using magnetic particle handling. Biotechniques. 43(3), pp 344-348.  [PubMed]

Wochner A., Glokler, J. (2007) Nonradioactive fluorescence microtiter plate assay monitoring aptamer selections. Biotechniques. 42(5), pp 578-582.  [PubMed]

Menger M., Glokler J., Rimmele M. (2006) Application of aptamers in therapeutics and for small-molecule detection. In: Erdmann V.A., Brosius J., Barciszewski J. (Eds.) RNA Towards Medicine. Handb Exp Pharmacol. 173, pp 359-373.  [PubMed]

Rimmele M., Ehrentreich-Forster E. (2004) Nukleinsäuren als biochemische Fängermoleküle. BIOforum 04, pp 68-69.  [PDF]

Rimmele M. (2003) Nucleic acid aptamers as tools and drugs: recent developments. Chembiochem. 4(10), pp 963-971.  [PubMed]

 

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