Rapid expression of recombinant proteins in high yields
EasyXpress Protein Synthesis Kit
- Get up to 1 mg protein per ml reaction volume in just one hour
- Compatible with phage T7 or E. coli T5 promotor containing vectors for E. coli expression
- A convenient procedure - pre-aliquoted extracts, color-coded reagents, and easy-to-follow protocols
- Fast procedure, ideally suited for rapid screening of expression templates - go from gene to protein in a single day in combination with the EasyXpress Linear Template Kit Plus
- For 5 x 50 µl reactions or 20 x 50 µl reactions: E. coli extract, reaction buffer, RNase-free water, positive-control DNA and handbook
Synthesis of recombinant posttranslationally modified eukaryotic proteins
EasyXpress Insect Kit II
- Robust expression of a wide range of proteins with a range of modifications - e.g., clotting factors, protein kinases, membrane proteins, transcription factors, and in particular functionally active glycoproteins
- Fast screening and increased flexibility - using PCR products or circular plasmids as expression templates
- Get up to 50 µg/ml, guaranteeing enough protein for functional studies and screening procedures
- For 5 x 50 µl reactions or 20 x 50 µl reactions: Spodoptera frugiperda insect cell extract, reaction buffers, in vitro transcription reaction components, RNase-free water, gel-filtration columns, positive-control DNA and handbook
Synthesis of recombinant proteins containing a single site-specifically incorporated biotin tag
EasyXpress Site-Specific Biotin Kit
- Synthesize biotin-tagged proteins in one step and use the advantages of the strong biotin-streptavidin (avidin, streptactin) interaction for your application – high affinity, small tag size and broad range of available detection reagents
- Tag placement at any desired position with a 1:1 stoichiometry - labeling distant from interaction sites
- Cotranslational incorporation of the Biotin-tag as single biotinylated amino acid (biotinylated lysine) encoded within the protein gene by a single codon (amber, UAG) using a highly efficient biotinylated amber suppressor tRNA in a specialised cell-free protein biosynthesis system
- Up to 5 µg (or 150 pmol) biotin-tagged protein per 25 µl reaction
- Lysate free of endogenous biotinylated proteins - enabling direct high affinity immobilization of synthesized biotin-tagged proteins
- A convenient procedure - pre-aliquoted extracts, color-coded reagents, and easy-to-follow protocols
- Compatible with the EasyXpress Linear Template Kit Plus and the Linear Template Kit Site-specific Label
- For 5 x 25 µl reactions: E. coli extract, reaction buffer, RNase-free Water, biotinyl-lysyl tRNA (amber), positive-control DNA and handbook
RiNAs Supplement Kits
Additives for cell-free translation reactions
- special amino acid mixtures, e.g. for NMR
- optimization components for better folding of proteins, e.g. Brij 25
- Single modified amino acids
- other
Please don't hesitate to contact us and ask us according to your special requirements