• From open reading frames (ORFs) to proteins in one day – generate templates for highly efficient protein expression in E. coli-based systems
• Addition of T7 promotor, T7 gene 10 sequence including ribosomal binding site and elements for mRNA stabilization using an optimized PCR system
• Generation of templates without tag or with N-/C-terminal Strep-/HisTag and optional N-terminal translation initiation enhancer sequence according to your requirements, cloning option
• Generated templates are also fully functional with the EasyXpress Insect Kit II
• For 20 two-step PCRs: Optimized PCR primers, ProofStart DNA Polymerase, buffers, positive-control DNA and handbook
  
  

Linear Template Kit N-terminal Label

• From open reading frames (ORFs) to proteins in one day – generate templates for highly efficient expression of site-specifically labeled proteins in E. coli-based systems
• Introduction of N-terminal expression enhancer-sequence containing in frame amber codon for efficient expression of site-specifically labeled proteins, e.g. using the EasyXpress Site-Specific Biotin Kit
• Addition of T7 promotor, T7 gene 10 sequence including ribosomal binding site and elements for mRNA stabilization using an optimized PCR system, cloning option
• C-terminal HisTag and no tag-options included
• For 20 two-step PCRs: optimized PCR primers, positive-control DNA and positive-control suppressor tRNA

EasyXpress pIX3.0 Vector

• Scaled up in vitro or in vivo expression using optimized templates
• 25 µg vector DNA

For detailed information refer to the EasyXpress Linear Template Kit Plus Handbook

EasyXpress pIX4.0 Vector

• Multiple cloning site and translational stop codons in all reading frames for convenient preparation of expression constructs for use in insect cell-based expression systems
• Efficient initiation of translation through a T7 promoter element combined with an optimized 3' UTR containing a T7 terminator for generation of stabilized RNA protected from degradation by exonucleolytic nucleases
• Site for plasmid linearization consisting of multiple restriction sites for blunt end linearization for effective in vitro transcription
• 25 µg vector DNA

For detailed informations refer to the EasyXpress Insect Kit Handbook

pIX5.0 Vector

• For T7 promotor-based protein expression in scaled-up in vitro translation reactions or in vivo systems
• Multiple cloning site and translational stop codons in all reading frames for convenient preparation of expression constructs
• 25 µg vector DNA