Synthesis of recombinant proteins containing a single site-specifically incorporated biotin tag
EasyXpress Site-Specific Biotin Kit
- Synthesize biotin-tagged proteins in one step and use the advantages of the strong biotin-streptavidin (avidin, streptactin) interaction for your application – high affinity, small tag size and broad range of available detection reagents
- Tag placement at any desired position with a 1:1 stoichiometry - labeling distant from interaction sites
- Up to 5 µg (or 150 pmol) biotin-tagged protein per 25 µl reaction
- Lysate free of endogenous biotinylated proteins - enabling direct high affinity immobilization of synthesized biotin-tagged proteins
- A convenient procedure - pre-aliquoted extracts, color-coded reagents, and easy-to-follow protocols
- Compatible with the EasyXpress Linear Template Kit Plus and the Linear Template Kit Site-specific Label
- For 5 x 25 µl reactions: E. coli extract, reaction buffer, RNase-free Water, biotinyl-lysyl tRNA (amber), positive-control DNA and handbook