Highly sensitive detection of cell-free expressed recombinant proteins via cotranslationally incorporated biotin
EasyXpress Random Biotin Kit
- Biotin labeling of proteins during cell-free synthesis for sensitive protein detection following western-blotting
- Compatible with all eukaryotic and prokaryotic cell-free protein biosynthesis systems, e.g. E. coli-, insect- or reticulocyte-based systems
- Efficient cotranslational biotin incorporation via precharged biotinylated tRNA using the frequent phenylalanine codon - guaranteeing efficient detection
- For 60 x 50 µl reactions: Biotinyl-lysyl tRNA (Phe)
For information regarding the use of the EasyXpress Random Biotin Kit with E. coli-based translation systems please refer to the EasyXpress Protein Synthesis Handbook, if using with eukaryotic translation systems please refer to the EasyXpress Insect Cell Protein Synthesis Handbook
Rapid and sensitive in gel-detection of cell-free expressed proteins
Coming soon: Random Fluorescence Labeling Kit
- Efficient labeling of proteins during cell-free protein synthesis for fluorescence-based in gel-detection directly after PAGE using a standard UV table
- No western blotting or radioactiv imaging required
- Alternatively, highly sensitive detection of proteins via Fluorescence Imaging (Excitation 532 nm)
- Compatible with insect- and E. coli-based EasyXpress protein synthesis kits
- Efficient cotranslational fluorescence-incorporation via precharged tRNA using the frequent phenylalanine codon - randomly labeled proteins develop as molecular tracer
- For 250 µl and for 1 ml translation reaction